Entries by DOE-MBI Staff

Protocols for some Crystallization Stock Solutions

Dan Anderson We have our own water machine to make HPLC grade water. Use this very pure water, and chemicals of ACS grade or better. Ammonium Sulfate, saturated solution: Weigh about 80 grams of enzyme grade ammonium sulfate. In a 250ml beaker, stir the solid into 100ml of wa ter. Heat the mixture to dissolve […]

DMM Accident

Chemical & Engineering News May 12, 1997 CHEMICAL SAFETY Handling dimethylmercury We report a case of severe mercury toxicity resulting from a single exposure to dimethylmercury. Review of research notes interviews, hair analysis, and statements made by the patient established the circumstances and events described here. Testing of the type of gloves worn by the […]

Protocols for some Crystallization Stock Solutions

Dan Anderson We have our own water machine to make HPLC grade water. Use this very pure water, and chemicals of ACS grade or better. Ammonium Sulfate, saturated solution: Weigh about 80 grams of enzyme grade ammonium sulfate. In a 250ml beaker, stir the solid into 100ml of wa ter. Heat the mixture to dissolve […]

DMM Safety Sheet

  University of California – MSDS System PAGE 1 05/16/95 Source of MSDS: VWR SCIENTIFIC CORPORATION MATERIAL SAFETY DATA SHEET EASTMAN KODAK COMPANY 343 STATE STREET ROCHESTER, NEW YORK 14650 FOR EMERGENCY HEALTH, SAFETY, AND ENVIRONMENTAL INFORMATION, CALL 716-722-51 FOR ALL OTHER PURPOSES, CALL 800-225-5352, IN NEW YORK STATE CALL 716-458-40 DATE OF PREPARATION: 08/22/86 […]

De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure

Publication in Nature, view here Abstract BinAB is a naturally occurring paracrystalline larvicide distributed worldwide to combat the devastating diseases borne by mosquitoes. These crystals are composed of homologous molecules, BinA and BinB, which play distinct roles in the multi-step intoxication process, transforming from harmless, robust crystals, to soluble protoxin heterodimers, to internalized mature toxin, […]

Eisenberg lab produces Ab Initio protein structure from prion nanocrystals at atomic resolution by MicroED

We illustrate a method to access the long unrealized potential of electrons in the crystallographic structural determination of biological molecules. Electrons, owing to their strong interaction with matter, produce high resolution diffraction patterns from tiny three-dimensional biomolecular crystals (MicroED), enticing structural biologists with a means to gain much needed insight into the detailed workings of […]

Todd Yeates work highlighted in Science

Todd Yeates lab published in Science and highlighted on the cover: Accurate design of megadalton-scale two-component icosahedral protein complexes. Included is a Video from Science Abstract Nature provides many examples of self- and co-assembling protein-based molecular machines, including icosahedral protein cages that serve as scaffolds, enzymes, and compartments for essential biochemical reactions and icosahedral virus capsids, which encapsidate and […]

UCLA Scientists Test New Strategy That Could Help Fight Ovarian Cancer

  Approach borrows from technique of inhibiting protein fibers that cause Alzheimer’s and Parkinson’s diseases * High-grade serous ovarian cancers are aggressive tumors, and most patients relapse despite standard therapy * UCLA researchers developed a peptide that penetrates cancer cells and stops their growth by restoring the protective function of the p53 protein * Scientists […]