Entries by DOE-MBI Staff

Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks

Interactions of LARKS protein domains More than 1500 human proteins contain long, disordered stretches of “low complexity”—strings of just a few of the 20 common amino acids. The functions of these low-complexity domains have been unclear. Hughes et al. present atomic-resolution structures that suggest that short segments of two such domains can bind weakly to […]

Low-complexity domains adhere by reversible amyloid-like interactions between kinked β-sheets

Control of metabolism by compartmentation is a widespread feature of higher cells. Recent studies have focused on dynamic intracellular bodies such as stress granules, P-bodies, nucleoli, and metabolic puncta. These bodies appear as separate phases, some containing reversible, amyloid-like fibrils formed by interactions of low-complexity protein domains. Here we report five atomic structures of segments […]

Structure-based inhibitors of tau aggregation

Published in Nature Chemistry Aggregated tau protein is associated with over 20 neurological disorders, which include Alzheimer’s disease. Previous work has shown that tau’s sequence segments VQIINK and VQIVYK drive its aggregation, but inhibitors based on the structure of the VQIVYK segment only partially inhibit full-length tau aggregation and are ineffective at inhibiting seeding by […]

Protocols for some Crystallization Stock Solutions

Dan Anderson We have our own water machine to make HPLC grade water. Use this very pure water, and chemicals of ACS grade or better. Ammonium Sulfate, saturated solution: Weigh about 80 grams of enzyme grade ammonium sulfate. In a 250ml beaker, stir the solid into 100ml of wa ter. Heat the mixture to dissolve […]

De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure

Publication in Nature, view here Abstract BinAB is a naturally occurring paracrystalline larvicide distributed worldwide to combat the devastating diseases borne by mosquitoes. These crystals are composed of homologous molecules, BinA and BinB, which play distinct roles in the multi-step intoxication process, transforming from harmless, robust crystals, to soluble protoxin heterodimers, to internalized mature toxin, […]

Eisenberg lab produces Ab Initio protein structure from prion nanocrystals at atomic resolution by MicroED

We illustrate a method to access the long unrealized potential of electrons in the crystallographic structural determination of biological molecules. Electrons, owing to their strong interaction with matter, produce high resolution diffraction patterns from tiny three-dimensional biomolecular crystals (MicroED), enticing structural biologists with a means to gain much needed insight into the detailed workings of […]

Todd Yeates work highlighted in Science

Todd Yeates lab published in Science and highlighted on the cover: Accurate design of megadalton-scale two-component icosahedral protein complexes. Included is a Video from Science Abstract Nature provides many examples of self- and co-assembling protein-based molecular machines, including icosahedral protein cages that serve as scaffolds, enzymes, and compartments for essential biochemical reactions and icosahedral virus capsids, which encapsidate and […]