Services provided by the Facility include: aid in crystallization; X-ray characterization of crystals; X-ray data collection in-house and at the synchrotron, processing and quality analysis of data; and structure determination and display.
Services/technologies provided by X-ray Crystallography Core Facility:
- Providing experimental and computational facilities for X-ray based structure analysis and refinement, and for supporting structure determination by crystallography, and computational methods.Acquisition of X-ray diffraction data using in-house high brilliance generators.
- Acquisition of Multi-wavelength Anomalous Dispersion data using synchrotron radiation
- X-ray diffraction data processing and manipulation.
- Assistance with the macromolecular model building and structure refinement processes.
- Guidance in the interpretation of the resulting three-dimensional macromolecular structures.
- Web-based services that include tutorials and software tools to assist users.
- Workshops and informal classes introducing the fundamentals of single crystal X-ray diffraction work to potential users. These workshops also provide an overview for how the core functionsand how users may interface with Core personnel.
- Micro crystallography: rapid screening of micro-crystals with the UV microscope. Manipulation and dissection of micro-crystals with a micro-manipulator.
To recover the cost of the supporting personnel, consumables and service contracts for the equipment, a fee will be charged to researchers.
The fee structure is designed to divide the ongoing costs among anticipated users, based on usage of the facility.
This usage covers numerous activities: x-ray data collection, machine maintenance, data collection at home, travel to synchrotrons and data collection there, expert assistance with structure determination (e.g. molecular replacement, heavy atom phasing, refinement), coordinate deposition, structure interpretation and analysis, and figure and manuscript preparation.
A point system of a unit has been developed to assign usage to participating research groups. INTERNAL X-RAY SERVICE (UCLA) will be charged $90 per unit. EXTERNAL X-RAY SERVICE ( NON-UCLA) willbe charged $135 per unit. A PI should anticipate a charge of around 20 units per quarter to use every instrument, and materials available in the facility including: FRE+ X-ray generators, HTC image plate detectors, High Power Leica Microscopes with High Resolution CCD Cameras, Korima UV microscope, all Cryogenic Equipment, storage of crystals in Liquid Nitrogen Dewars, storage in the 5 degrees Crystallization Room, data collection at the synchrotron and experienced staff assistance with structure determination.
New users should discuss setting up a recharge account with the X-ray Core Director.