Entries by DOE-MBI Staff

Methanospirillum hungatei cells and flagella structure

The archaeal motility structure (flagellum /archaellum) During an initial structural and proteomic survey of the model archaeal microbe Methanospirillum hungatei we identified proteins comprising the attached cellular flagellum. Little was previously known about these archaeal appendages and we therefore characterized them and obtained the first atomic-level structure of a model archaeal flagellum. The findings reveal […]

Genome-wide transcriptomics in Chlamydomonas reinhardtii during day-night cycles reveals new metabolic patterns in light and stress response (Merchant lab)

A day in the life of Chlamydomonas The unicellular green alga Chlamydomonas reinhardtii excels at acclimating to a changing environment. We analyzed expression patterns of its three genomes in cells grown under light-dark cycles. Nearly 85% of transcribed genes show differential expression, with different sets of transcripts being up-regulated over the course of the day […]

Development of a new enzyme reagent that labels proteins with peptides via a specific lysine-isopeptide bond at high yield (Clubb lab)

Proteins that are site-specifically modified with peptides and chemicals can be used as novel therapeutics, imaging tools, diagnostic reagents and materials. However, few enzyme-catalyzed methods are currently available to selectively conjugate peptides to internal sites within proteins. To overcome this problem, the Clubb, Loo and Ton-That laboratories have developed a new enzyme reagent that labels […]