Entries by DOE-MBI Staff

Methanospirillum hungatei cells and flagella structure

The archaeal motility structure (flagellum /archaellum) During an initial structural and proteomic survey of the model archaeal microbe Methanospirillum hungatei we identified proteins comprising the attached cellular flagellum. Little was previously known about these archaeal appendages and we therefore characterized them and obtained the first atomic-level structure of a model archaeal flagellum. The findings reveal […]

Genome-wide transcriptomics in Chlamydomonas reinhardtii during day-night cycles reveals new metabolic patterns in light and stress response (Merchant lab)

A day in the life of Chlamydomonas The unicellular green alga Chlamydomonas reinhardtii excels at acclimating to a changing environment. We analyzed expression patterns of its three genomes in cells grown under light-dark cycles. Nearly 85% of transcribed genes show differential expression, with different sets of transcripts being up-regulated over the course of the day […]

Development of a new enzyme reagent that labels proteins with peptides via a specific lysine-isopeptide bond at high yield (Clubb lab)

Proteins that are site-specifically modified with peptides and chemicals can be used as novel therapeutics, imaging tools, diagnostic reagents and materials. However, few enzyme-catalyzed methods are currently available to selectively conjugate peptides to internal sites within proteins. To overcome this problem, the Clubb, Loo and Ton-That laboratories have developed a new enzyme reagent that labels […]

New method to create stable enzyme coated B. subtilis cells for biotechnological applications.

Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and already used industrially to produce enzymes. The Clubb group developed a unique two-step procedure that enables the […]

James Bowie was named Fellow of the Biophysical Society

James Bowie was named Fellow of the Biophysical Society and he delivered the Choh Hao Li Memorial Lecture at Academia Sinica. Other awards: 2018 Choh Hao Li Memorial Lecture, Academia Sinica 2018 UCLA Postdoctoral Society Postdoc Mentoring Award Finalist 2017 Anatrace Award for Membrane Proteins, Biophysical Society