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Lab Protocols

Lab Protocols

    • Fluorine Labeling Of Proteins For NMR Studies (by Robert Peterson)
    • Specific Labeling (Seleno Methionine) of Proteins (by Mahavir Singh)
    • Native His-Tag Purification (by Arthur Laganowsky)
    • Fiber Alignment with Glass Rods (by Michael Sawaya ala Magdalena Ivanova)
    • Another Se-Met Labeling Protocol (by Mark Arbing)
    • Colony PCR for pET vectors; Pichia pastoris electroporation (by Mark Arbing)
    • Proper Loading of Sample in AKTA (by James Stroud)
    • Glycerol concentrations for crystal Cryoprotection ( HAMPTON CS1)  (by Duilio Cascio)
    • Yet another Seleno-Methionine Protocol (by Andrew Min)
    • Making LB Broth (Miller) or Terrific Broth (by Arthur Laganowsky)
    • pBAC Transformation and Bacmid Purification for Insect Cells (by Arthur Laganowsky)

Lab Chemical Safety SOPs

  • Ammonium Nitrate (Dan Anderson)
  • Cyanogen Bromide (James Stroud)
  • Dansyl Chloride (Howard Chang)
  • Dichloromethane (Dan Anderson)
  • Formaldehyde (Dan Anderson)
  • Sodium Azide (Dan Anderson)
  • Weighing Dry Amyloidogenic Peptides (Dan Anderson)

Mail Address

611 Charles Young Drive East

Los Angeles, CA 90095

Campus Office Address

201 Boyer Hall Box 951570

Mail Code 157005

Phone: (310) 825-3754

Fax: (310) 206-3914


Biological and Environmental Research (DOE-BER)

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