Proper Loading of Sample in AKTA
First, a definition of terms:
"Loading" is the process of loading the sample into the loop--the
operator does this part manually in general.
"Injecting" is the process of the sample's being pumped from the loop
and into the column. The AKTA pumps take care of this part.
Confusion of these two terms is likely a major reason that
proper sample loading technique is frequently misunderstood.
To properly load sample from a syringe to the AKTA:
1. Clear all air bubbles from the sample in the syringe
(to protect sizing columns).
2. GENTLY insert the syringe into the syringe valve until
it stops. As Dan might say, you only need 0.001% finger
strength to do this. DO NOT press the syringe in as if you are
trying to puncture something. The seal is formed by the
blunt abutting of two surfaces, NOT by the pressure
of a punctured membrane surrounding the needle.
The surfaces only need to make contact to form a
seal. The needle does not puncture anything.
3. Leave the syringe and needle in place until the
sample is injected and the valve position moves
back to "load" or else you risk losing your
sample to gravity.
4. NEVER use a "syringe needle" with a pointed tip to
load the AKTA. The question you want to ask yourself
when loading the AKTA is: "Would I want what I am about to
do done to my inner ear?"