Consultation and technical assistance available to researchers prior to, and following, experiments.
Evaluation of sample via Dynamic Light Scattering.
Automatic setup of crystallization conditions at 4° and 20° C.
Storage of plates at 4° and 20° C.
UV/vis microscope distinguishes between organic and inorganic crystals.
Optimization of crystallization conditions.
The crystallization facility at UCLA is funded by the DOE and is the most cost-efficient means of high-throughput screening in the United States.
Internal UCLA researchers:
Crystallization tray with 96 commercial solutions, 3 drop experiment, 288 conditions: $35
Crystallization tray with 96 commercial solutions, 3 drop experiment, 288 conditions: $40
The Crystallization Facility has a large library of commercial kits on hand.
TTP LabTech Mosquito nanoliter-scale liquid handlers generate hanging and sitting drop crystallization experiments.
The three-drop pattern (pictured right) requires 40 ul of sample and produces 288 unique conditions 210 nl each. For initial screening we typically recommend a three-drop pattern that varies protein:chemical concentration to increase the chance of obtaining crystals.
After a sample is received it is prepared immediately. A total of 2,880 conditions only requires 400ul of sample.
The minimum volume required to set up a single drop experiment is 15 uL (100nl sample: 100nl crystallant).
A basic Requirement for macromolecular crystallization is sample purity better than 98% as detected by SDS gel and/or Mass Spectroscopy. The presence of secondary bands in the gels or other contaminants will decrease the success rate. The optimal protein concentration is around 10 mg/ml but other concentrations can be accepted as long as the technical staff is notified. The normal turn around is 24 hours. Special optimization conditions may require more time. We recommend to filter the sample shortly before the crystallization trials starts.
WYATT DynaPro Plate Reader II for evaluation of sample prior to setting crystallization plates.
TTP LabTech Mosquito nanodispenser robot capable of setting hanging and sitting drops at a rate of 288 conditions every 2.9 minutes.
Tecan Genesis 200 to prepare crystallization kits and to generate optimization conditions.
Korima PRS-1000 UV microscope capable of distinguishing organics from salt crystals.
Emerald BioSystems Crystal Monitor microscope workstation with automated image capture.
Tecan TEMO for mother-daughter replication and to dispense solutions from commercial kits.
Commercial Screen Formulations available in 96 well format.