Screens

AmSO4

Grid/sparse matrix/ionic.
Composition (PDF  |  Excel)

JCSG+

JCSG-plus is the screen of choice for initial screening experiments. The most complete sparse matrix screen available today.
Composition (PDF  |  Excel)

MPDs
Grid/sparse matrix/ionic, 2-methyl-2,4-pentandiol organic precipitant.
Composition (PDF  |  Excel)

PACT
Grid, pH/ionic screen with PEG as precipitant.
Composition (PDF  |  Excel)

PEG
Sparse matrix.
Composition (PEG  |  Excel: A1-D12, E1-H12)

ProPlex ECO
Formulated for the crystallization of protein complexes.
Composition (PDF  |  Excel)

Structure Screen (SS12)
Formulated for the crystallization of proteins, peptides, nucleic acids, & water soluble small molecules.
Composition (PDF  |  Excel)

Wizard
Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5
Composition (PDF  |  Excel)

Index
Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules.
Composition (PDF  |  Excel)

Limited Use

Natrix
Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes.
Composition (PDF  |  Excel)

Crystal Screen
Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules – sparse matrix additive screen.
Composition (PDF  |  Excel)

Midas Plus
Ideal for soluble protein, protein/protein complexes, protein-nucleic acid complexes and sensitive macromolecular complexes.
Composition (PDF  |  Excel)

MemGold
Sparse matrix, designed for alpha and beta membrane proteins.
Composition (PDF)

HELIX
Crystallize a diverse range of nucleic acid topologies and molecular weights. This screen targets all types of DNA/RNA, triplexes, quadruplexes, pseudoknots, i-motifs, and large molecular weight nucleic acids.
Composition (PDF  |  Excel)

Crystal Screen Cryo
Complete sparse matrix reagent kit designed to provide a rapid screening method for crystallization in the presence of glycerol.
Composition (PDF  |  Excel)

Nucleic Acid Mini Screen

Crystallization screen for nucleic acid fragments.
Composition (PDF  |  Excel)

MEMSTART/SYS – Out of Stock
A great starting point for membrane protein labs for screening and optimizing crystallization conditions for alpha – helical type transmembrane proteins.
Composition (PDF)

SaltRx – Out of Stock
Sparse matrix, salt & pH only.
Composition (PDF  |  Excel)

Additives

Additive Screen Optimization 
96 unique additives.
Composition (PDF)

Detergent Screen Optimization – Out of Stock
96 unique detergents, ideal for membrane proteins.
Composition (PDF)

Emerald Additive Screen Optimization – Out of Stock
96 unique additives.
Composition (PDF)

Preparation

Please prepare a recharge form before coming to the laboratoy.

Tools

HT Seeding (2019 Sobol, Collazo, and Johnson)

This poster displays an experiment in high-throughput format seeding. Seed crystal, 10 mL of reservoir solution, sample, and 200 uL of sample buffer are required to attempt this protocol. Contact the crystallization laboratory in advance for a consultation.
HT Seeding Poster

Optimization Maker (2017 Collazo and Johnson)

Design expansions of your hits in a 96-well format. You can make the plate easily using a multi-channel or bring the stocks to the laboratory with this sheet and the volumes will be compatible with our instrumentation.
Optimization Maker
 (Downloads Excel File)

Condition Finder Beta (2016 Collazo)

Save this file and easily identify conditions without downloading formulations or connecting to the internet. This file is undergoing continued validation by a group of users. Be sure to check with the formulation sheets until it is fully validated.
Condition Finder Beta (Downloads Excel File)

Screen comparison (2011 Jason Navarro)

If you’re curious about the amount of overlap between the screens below:
Crystallization Screen Comparison (Downloads Excel File)

Scoring older trays?

If you don’t see your screen listed, check here:
http://www.doe-mbi.ucla.edu/screenarchive (Link)

Crystallization Kits

The Sample Preparation and Crystal Screening Laboratory  utilizes commercial kits from Molecular Dimensions, and Hampton Research. For a reasonable chance of crystallizing a sample, screen 4-5 trays. For cytosolic protein we recommend, JCSG+, PACT, SS12, PEG, and WIZARD. If sample is severely limited, JCSG and PACT are recommended.

It is a good idea to contact Genesis Falcon beforehand to check availability. Email ucla.xtal.facility@gmail.com

Scoring Sheets

Additive Screen Scoring Sheet (A1-H12)

Index Scoring Sheet (A1-H12)

PEG Scoring Sheet (A1-H12)

MPD Scoring Sheet (A1-H12)

AmSO4 Scoring Sheet (A1-H12)

Natrix Scoring Sheet (A1-H12)

JCSG+ Scoring Sheet (A1-H12)

Crystal Screen Scoring Sheet (A1-H12)

Crystal Screen Cryo Scoring Sheet (A1-H12)

NAMS Scoring Sheet (A1-H12)

PACT Scoring Sheet (A1-H12)

SaltRx Scoring Sheet (A1-H12)

Detergent Screen Scoring Sheet (A1-H12)