Please prepare a recharge form before coming to the laboratoy.
HT Seeding (2019 Sobol, Collazo, and Johnson)
This poster displays an experiment in high-throughput format seeding. Seed crystal, 10 mL of reservoir solution, sample, and 200 uL of sample buffer are required to attempt this protocol. Contact the crystallization laboratory in advance for a consultation.
HT Seeding Poster
Optimization Maker (2017 Collazo and Johnson)
Design expansions of your hits in a 96-well format. You can make the plate easily using a multi-channel or bring the stocks to the laboratory with this sheet and the volumes will be compatible with our instrumentation.
Optimization Maker (Downloads Excel File)
Condition Finder Beta (2016 Collazo)
Save this file and easily identify conditions without downloading formulations or connecting to the internet. This file is undergoing continued validation by a group of users. Be sure to check with the formulation sheets until it is fully validated.
Condition Finder Beta (Downloads Excel File)
Screen comparison (2011 Jason Navarro)
If you’re curious about the amount of overlap between the screens below:
Crystallization Screen Comparison (Downloads Excel File)
Scoring older trays?
If you don’t see your screen listed, check here:
The Sample Preparation and Crystal Screening Laboratory utilizes commercial kits from Molecular Dimensions, and Hampton Research. For a reasonable chance of crystallizing a sample, screen 4-5 trays. For cytosolic protein we recommend, JCSG+, PACT, SS12, PEG, and WIZARD. If sample is severely limited, JCSG and PACT are recommended.
It is a good idea to contact Genesis Falcon beforehand to check availability. Email firstname.lastname@example.org
Additive Screen Scoring Sheet (A1-H12)
Index Scoring Sheet (A1-H12)
PEG Scoring Sheet (A1-H12)
MPD Scoring Sheet (A1-H12)
AmSO4 Scoring Sheet (A1-H12)
Natrix Scoring Sheet (A1-H12)
JCSG+ Scoring Sheet (A1-H12)
Crystal Screen Scoring Sheet (A1-H12)
Crystal Screen Cryo Scoring Sheet (A1-H12)
NAMS Scoring Sheet (A1-H12)
PACT Scoring Sheet (A1-H12)
SaltRx Scoring Sheet (A1-H12)
Detergent Screen Scoring Sheet (A1-H12)