Dynamic light scattering helps determine whether a sample is selective for a single oligomeric state, or if problems such as impurity or aggregation are present.
How do I prepare a sample?
Contact Michael Collazo at email@example.com or (310) 882-2585 to set up a time to run the experiments before preparing your samples. On the day of your appointment, filter your sample and set aside 30 uL per sample that you would like to measure (1 to 10 samples is common). Filtration through a 0.2 micron filter is required just prior to arriving with samples. Do not filter if your sample contains detergent. Your samples should be at least 1 to 2 mg/ml in concentration. The DLS is useful for samples 0.5 to 1,000 nanometers in diameter.
You will be trained to load the DLS, use the software, troubleshoot, interpret data, and save data as an XPS file (45-60 minute training). You can bring a USB stick or email the data to yourself. Once you are proficient, you will receive the password to the DLS to work independently.
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