FAQ for Crystallization Setups
Why are crystallization setups performed by the X-ray and EM structure determination core?
Setting up crystallization experiments by hand consumes time and sample. The crystallization core uses a nanodispenser called the STP Mosquito to make hanging-drop trays containing 288 unique conditions that may crystallize a sample. The automated setup of a single tray of 288 conditions only takes three minutes.
How do I prepare a sample?
Contact Genesis Falcon at the Sample Preparation and Crystal Screening Laboratory (BH-106 or firstname.lastname@example.org) to set up a time to run the experiments before preparing your samples. Once you have an appointment, set aside 40 uL per sample that you would like to crystallize (2-5 trays is common). Refer to literature or ask for a recommendation when selecting a sample concentration. We highly recommend a 0.2 micron filter immediately before running the sample for best results.
You will be trained to identify crystals using a stereo microscope. Use a sharpie to mark wells of interest and clearly circle wells with crystals so staff can easily assist you with the crystals. Most crystals grow in 2-3 days. Some take months to appear. Ask for advice and check in with Genesis Falcon to best manage your experiment.