Notes and protocols

Electrophoresis

• Gel receipes
• SDS-PAGE notes
• Native PAGE notes
• Alternative staining methods
  • Silver stain
  • Zinc/imidazole negative stain

Protein conjugation

• Alkylating free thiols
• Glutaraldehyde protein crosslinking

Protein prep

• Inclusion body prep
• AF20xx protein prep
• PDO prep
• TEV purification
• Preparative PAGE
  • BAC/Acrylamide dissolvable gels
  • Bio-rad 491 Prep gel unit
  • Electroelution of excised bis/Acrylamide gels

Miscellaneous

• Ethanol precipitation
• Buffer quick ref
• TEV protease cleavage

Equipment usage notes

• Hitachi H-7000 Electron microscope
• Imacon negative scanner

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morgan(at)mbi.ucla.edu