Protein Expression

pET Vectors

Vector

Features

Resistance

Notes

pET3a, 3b, 3c, 3d

pBR322 origin origin, no tag

Ampicillin

New England Biolabs

pET15b

pBR322 origin, lacI, N-terminal His6, thrombin protease site

Ampicillin

Novagen

pET16b

pBR322 origin, lacI, N-terminal His6, Factor Xa protease  site

Ampicillin

Novagen

pET17b

pBR322 origin, N-terminal T7 tag

Ampicillin

Novagen

pET22b

pBR322 origin, lacI, N-terminal PelB leader with signal peptidase site, C-terminal His6

Ampicillin

Novagen

pET23a, 23b, 23c, 23d

pBR322 origin, N-terminal T7 tag, C-terminal His6

Ampicillin

Novagen

pET26b

pBR322 origin, lacI, N-terminal PelB leader with signal peptidase site, C-terminal His6

Kanamycin

Novagen

pET28a, 28b, 28c

pBR322 origin, lacI, N-terminal His6, thrombin protease site, C-terminal His6

Kanamycin

Novagen

pET32a, 32b, 32c

pBR322 origin, lacI, N-terminal thioredoxin, His6, thrombin site, S-tag, enterokinase protease site, C-terminal His6

Ampicillin

Novagen

pET39b

pBR322 origin, lacI, N-terminal DsbA fusion, His6, thrombin, S-tag, Enterokinase site, C-terminal His6

Kanamycin

Novagen

pET40b

pBR322 origin, lacI, N-terminal DsbC fusion, His6, thrombin, S-tag, Enterokinase protease site, C-terminal His6

Kanamycin

Novagen

pET-M11

pBR322 origin, lacI, N-terminal His6, TEV protease site, C-terminal His6

Kanamycin

EMBL

pET-M20

pBR322 origin, lacI, Thioredoxin, His6, TEV protease site, C-terminal His6

Ampicillin

EMBL

pET-M30

pBR322 origin, lacI, N-terminal His6, GST, TEV protease site, C-terminal His6

Kanamycin

EMBL

pET-M40

pBR322 origin, lacI, MBP, TEV protease site, C-terminal His6

Kanamycin

EMBL

pET Co-expression Vectors

pET-DUET-1

pBR322 origin, lacI, MCS1 = N-terminal His6, MCS2 = C-terminal S-tag

Ampicillin

Novagen

pRSFDuet-1

lacI, RSF origin, MCS1= N-terminal His6, MCS2 = C-terminal S-tag

Kanamycin

Novagen

pCDFDuet-1

lacI, CDF origin, MCS1= N-terminal His6, MCS2 = C-terminal S-tag

Streptomycin, spectinomycin

Novagen

Our LIC vectors (T7 and pBAD promoters)

Other Vectors

pTrcHisA/B/C

pBR322 origin, trc promoter, lacIq, N-terminal His6, Xpress tag, enterokinase protease site

Ampicillin

Invitrogen

pTrxFus

ColE1 origin, Pl promoter, N-terminal thioredoxin fusion, enterokinase protease site

Ampicillin

Invitrogen

pGEX-4T-1

pBR322 origin, N-terminal GST fusion, thrombin protease site, Ptac promoter, lacIq

Ampicillin

GE Healthcare

pQE-1

ColE1 origin, T5 promoter, N-terminal His6, TagZyme

Ampicillin

Qiagen

pQE-2

ColE1 origin, T5 promoter, N-terminal His6, TagZyme

Ampicillin

Qiagen

pQE-30

ColE1 origin, T5 promoter, N-terminal His6

Ampicillin

Qiagen

pMAL-C2X

Ptac promoter, lacIq, cytoplasmic N-terminal MBP fusion, Factor Xa protease site

Ampicillin

New England Biolabs

pMAL-P2E

Ptac promoter, lacIq, periplasmic N-terminal MBP fusion, enterokinase site

Ampicillin

New England Biolabs

pMAL-C2

pBR322 origin, Ptac, lacIq, cytoplasmic MBP fusion, Factor Xa protease site

Ampicillin

New England Biolabs

pMAL-P2

Ptac, lacIq, periplasmic MBP fusion, enterokinase protease site

Ampicillin

New England Biolabs

pCOLD

lacI, cspA promoter for ColEI, N-terminal His6, Factor Xa protease site

Ampicillin

Takara

Gateway™ Vectors

pKM596

Cytoplasmic N-terminal MBP fusion.

Ampicillin

David Waugh

pMA70

Periplasmic N-terminal MBP fusion.

Ampicillin

 PETC

pDEST17

Cytoplasmic N-terminal His6 tag

Ampicillin

Invitrogen

pDONR221

Entry vector

Kanamycin

Invitrogen

pGT-TRX

Cytoplasmic N-terminal Thioredoxin fusion

Ampicillin

PETC

pGT-DsbA

Cytoplasmic N-terminal DsbA fusion

Ampicillin

PETC

pGT-DsbC

Cytoplasmic N-terminal DsbC fusion

Ampicillin

PETC

pBAD-DEST49

pUC origin, cytoplasmic TRX, EK site, Gateway cloning site, C-terminal His6. pBAD promoter.

Ampicillin

Invitrogen

Vector

Features

Resistance

Source

pPICZα-A

P. pastoris.

Zeocin

Invitrogen

pPICZ-A, -C

P. pastoris.

Zeocin

Invitrogen

pGT-pPICZα

P. pastoris. Gateway vector.

Zeocin

pHIL-D2

P. pastoris.Shuttle/expression vector, pBR322 origin, AOX1 promoter, 8.2 kb

Ampicillin, His4

Invitrogen

pHIL-S1

P. pastoris. Shuttle/expression vector, pBR322 origin, AOX1 promoter, 8.3 kb, PHO1 signal sequence for secretion

Ampicillin, His4

Invitrogen

pPIC9

P. pastoris. Shuttle/expression vector, pBR322 origin, AOX1 promoter, 8.0 kb, α-factor signal sequence for secretion

Ampicillin, His4

Invitrogen

pPIC9K

P. pastoris. pBR322 origin, α-factor signal sequence for secretion, AOX1 promoter

Ampicillin, His4, kanamycin

Invitrogen

pAO815

P. pastoris. pBR322 origin, AOX promoter, 7.7 kb.

Ampicillin, His4

Invitrogen

pYES2

S. cerevisiae. pGAL1 promoter, pUC origin, 5.9 kb.

Ampicillin, URA3

Invitrogen

Vector

Features

Resistance

Source

pRK603

Allows for in vivo cleavage of fusion proteins with a TEV protease site

Kanamycin

David Waugh

pRK1037

Allows for in vivo cleavage of fusion proteins with a TVMV protease site

Kanamycin

David Waugh

pBluescript SK+

pUC origin, cloning vector

Ampicillin

Stratagene

pGroESL

p15A origin. Vector for chaperone co-expression

Chloramphenicol

Goloubinoff et al., 1989, Nature

pG-KJE8

L-Arabinose, tetracyclin, dnaK-dnaJ-grpE-groES-groEL. Vector for chaperone co-expression

Chloramphenicol

Takara

pGro7

L-Arabinose, groES-groEL. Vector for chaperone co-expression

Chloramphenicol

Takara

pKJE7

L-Arabinose, dnaK-dnaJ-grpE. Vector for chaperone co-expression

Chloramphenicol

Takara

pG-Tf2

Tetracyclin, groES-groEL-tig. Vector for chaperone co-expression

Chloramphenicol

Takara

pTf16

L-Arabinose, tig. Vector for chaperone co-expression

Chloramphenicol

Takara