Crystallization Core
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Trials, Optimizations, Appointments | Billing Info, New Users, Screens Available
Commercial Crystallization Kits
The Crystallization Facility utilizes commercial kits from Emerald Biosystems, Hampton Research, and Qiagen. For initial screening of samples we typically recommend 4-8 screens consisting of: Crystal Screen 1-2, Index, JCSG+, PACT, PEG-ion/Cryo, SaltRx, Wizard 1-2, and AmSO4.
Please note, only underlined screens are kept ready to use for crystallization trials. You must contact me well ahead of time in order to use any other screen.
If you're curious about the amount of overlap between the screens below, download this (excel):
Crystallization Screen Comparison
Download Composition Tables & Scoring Sheets
Emerald Biosystems
www.emeraldbiosystems.com
ADDit Additive Screen (EBadd) Optimization
96 unique additives
Composition (pdf)
Wizard I+II (wiz, wiz1-2)
sparse matrix, broad screen of buffers, crystallants, and pH
Composition (pdf), Scoring Sheet (pdf)
Hampton Research
www.hamptonresearch.com
Additive Screen (HRadd) Optimization
96 unique additives
Composition (pdf), Scoring Sheet (pdf)
Crystal Screen I + Crystal Screen II (cs, cs1-2)
sparse matrix, broad screen of salts, polymers, organics, and pH
Composition (pdf), Scoring Sheet (pdf)
Detergent Screen (HRdet) Optimization
96 unique detergents, ideal for membrane proteins
Composition (pdf), Scoring Sheet (pdf)
Index (ind)
grid, sprase matrix, broad screen of salts, pH, PEG, and organics
Composition (pdf), Scoring Sheet (pdf)
PEG/Ion + Crystal Screen Cryo (pegion/cryo, PIC, PEG)
sparse matrix
Composition (pdf, pdf), Scoring Sheet (pdf, pdf)
SaltRx (salt)
sparse matrix, salt & pH only
Composition (pdf), Scoring Sheet (pdf)
Silver Bullets (sb) Optimization
96 solutions using small molecule/macromolecular digests
Composition (pdf), Scoring Sheet (pdf)
Qiagen
www.qiagen.com
AmSO4 (Am, AS) New
grid/sparse matrix/ionic
Composition (pdf), Scoring Sheet (pdf)
ComPAS (com, comp)
sparse matrix, salts/organics/polymers as precipitants
Composition (pdf), Scoring Sheet (pdf)
Cryos (cryo)
sparse matrix, glycerol as cryoprotectant
Composition (pdf), Scoring Sheet (pdf)
JCSG+ (jcsg)
sparse matrix, mostly polymers as preciptitant
Composition (pdf), Scoring Sheet (pdf)
JCSG Core Suites I-IV (jcsg1-4) New
conditions giving the most hits at the Joint Center for Structural Genomics
Composition (pdf,pdf,pdf,pdf), Scoring Sheet (pdf,pdf,pdf,pdf)
MbClass (mb1)
sparse matrix, designed for membrane proteins
Composition (pdf), Scoring Sheet (pdf)
MbClass II (mb2)
sparse matrix, designed using conditions of alpha-helical proteins
Composition (pdf), Scoring Sheet (pdf)
MPDs (mpd) New
grid/sparse matrix/ionic, 2-methyl-2,4-pentandiol organic precipitant
Composition (pdf), Scoring Sheet (pdf)
Nucleix (nuc)
sparse matrix, designed for nucleic acid/protein-nucleic acid complexes
Composition (pdf), Scoring Sheet (pdf)
PACT (pa)
grid, pH/ionic screen with PEG as precipitant
Composition (pdf), Scoring Sheet (pdf)
ProComplex (pro, procom)
sparse matrix, designed for protein-protein complexes
Composition (pdf), Scoring Sheet (pdf)
