FACILITIES

Crystallization Core

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Commercial Crystallization Kits

The Crystallization Facility utilizes commercial kits from Emerald Biosystems, Hampton Research, and Qiagen. For initial screening of samples we typically recommend 4-8 screens consisting of: Crystal Screen 1-2, Index, JCSG+, PACT, PEG-ion/Cryo, SaltRx, Wizard 1-2, and AmSO4.

If you're curious about the amount of overlap between the screens below, download this (excel):
Crystallization Screen Comparison

Download Composition Tables & Scoring Sheets

UCLA
    Inna's Screen (inn)
        sparse matrix, broad screen using mostly polymers as precipitants
        Composition (pdf), Scoring Sheet (pdf)

Emerald Biosystems
    ADDit Additive Screen (EBadd) New, Optimization
        96 unique additives
        Composition (pdf, xls)
    Wizard I+II (wiz, wiz1-2)
        sparse matrix, broad screen of buffers, crystallants, and pH
        Composition (pdf, xls), Scoring Sheet (pdf)

Hampton Research
    Additive Screen (HRadd) New, Optimization
        96 unique additives
        Composition (pdf, xls), Scoring Sheet (pdf)
    Crystal Screen I + Crystal Screen II (cs, cs1-2)
        sparse matrix, broad screen of salts, polymers, organics, and pH
        Composition (pdf, xls), Scoring Sheet (pdf)
    Detergent Screen (HRdet) New, Optimization
        72 unique detergents, ideal of membrane proteins
        Composition (pdf, xls), Scoring Sheet (pdf)
    Index (ind)
        grid, sprase matrix, broad screen of salts, pH, PEG, and organics
        Composition (pdf, xls), Scoring Sheet (pdf)
    PEG/Ion + Crystal Screen Cryo (pegion/cryo, PIC, PEG)
        sparse matrix
        Composition (pdf, xls), Scoring Sheet (pdf)
    SaltRx (salt)
        sparse matrix, salt & pH only
        Composition (pdf, xls), Scoring Sheet (pdf)

Qiagen
    AmSO4 (Am, AS) New
        grid/sparse matrix/ionic
        Composition (pdf, xls), Scoring Sheet (pdf)
    Classics (clas)
        sparse matrix, identical to Cryos (without glycerol)
        Composition (pdf, xls), Scoring Sheet (pdf)
    ComPAS (com, comp)
        sparse matrix, salts/organics/polymers as precipitants
        Composition (pdf, xls), Scoring Sheet (pdf)
    Cryos (cryo)
        sparse matrix, glycerol as cryoprotectant
        Composition (pdf, xls), Scoring Sheet (pdf)
    JCSG+ (jcsg)
        sparse matrix, mostly polymers as preciptitant
        Composition (pdf, xls), Scoring Sheet (pdf)
    JCSG Core Suites I-IV (jcsg1-4) New
        conditions giving the most hits at the Joint Center for Structural Genomics
        Composition (pdf - I, II, III, IV, xls), Scoring Sheet (pdf - I, II, III, IV)
    MbClass (mb1)
        sparse matrix, designed for membrane proteins
        Composition (pdf, xls), Scoring Sheet (pdf)
    MbClass II (mb2)
        sparse matrix, designed using conditions of alpha-helical proteins
        Composition (pdf, xls), Scoring Sheet (pdf)
     MPDs (mpd) New
        grid/sparse matrix/ionic, 2-methyl-2,4-pentandiol organic precipitant
        Composition (pdf, xls), Scoring Sheet (pdf)
    Nucleix (nuc)
        sparse matrix, designed for nucleic acid/protein-nucleic acid complexes
        Composition (pdf, xls), Scoring Sheet (pdf)
    PACT (pa)
        grid, pH/ionic screen with PEG as precipitant
        Composition (pdf, xls), Scoring Sheet (pdf)
    ProComplex (pro, procom)
        sparse matrix, designed for protein-protein complexes
        Composition (pdf, xls), Scoring Sheet (pdf)