Crystallization Core
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Trials, Optimizations, Appointments | Billing Info, New Users, Screens Available
Optimization Trays
The Crystallization Facility is able to design customized screens based on hits from initial screening with commercial kits. We utilize OptiCrys to design the optimization plate and generage an excel file of liquid values, which is imported into our Tecan Genesis pipetting robot.
Two options for creating your optimization
The facility sets up the optimization for you, and creates the plate
This process involves some discussion of the desired optimization parameters, and all pipetting values are setup using OptiCrys in the facility. We then use the facility's robot to pipett the liquids for you, and then setup the crystallization experiment.
You set up your own optimization, and use the facility to create the plate
Alternatively, you may setup the optimization parameters, create an excel file with all pipetting values, and simply use the facility's robot to pipett the liquids for you. We then setup the crystallization experiment using the optimizatin plate.
If considering this route, please be aware of the following:
- the robot is only able to pipett accurately >10ul
- the robot is unable to pipett high % PEG stock solutions
- OptiCrys does not correctly set up pH gradients
- column names cannot have special characters, spaces, underscores, or dashes
Please format your excel file like the following:
| Well | Salt | Precipitant | Buffer | Water |
| A1 | 15 | 20 | 25 | 40 |
| A2 | 17 | 20 | 25 | 38 |
| A3 | 19 | 20 | 25 | 36 |
| A4 | 21 | 20 | 25 | 34 |
| A5 | 23 | 20 | 25 | 32 |
What we need to make your optimization
► Any chemical reagents to be used in the optimization plate, preferably in 15ml centrifuge tubes (this includes water).
► Signed billing form.
► At least 20ul of sample (this translates to 200nl of sample/drop).
► Any special instructions about your sample.
